The role of lipoylation in mitochondrial adaptation to methionine restriction.

Dietary methionine restriction (MR) is associated with a spectrum of health-promoting benefits. Being conducive to prevention of chronic diseases and extension of life span, MR can activate integrated responses at metabolic, transcriptional, and physiological levels. However, how the mitochondria of MR influence metabolic phenotypes remains elusive. Here, we provide a summary of cellular functions of methionine metabolism and an overview of the current understanding of effector mechanisms of MR, with a focus on the aspect of mitochondria-mediated responses. We propose that mitochondria can sense and respond to MR through a modulatory role of lipoylation, a mitochondrial protein modification sensitized by MR.

Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS.

We describe a protocol for measuring phospholipid class and fatty acid composition in the budding yeast Saccharomyces cerevisiae using a liquid chromatography-mass spectrometry (LC-MS)-based approach. We compile a mass spectral-retention time library verified for major phospholipids in the budding yeast and showcase the profiling process of phospholipid compositions in mutants with defective syntheses of phosphatidylethanolamine (PE) and phosphatidylcholine (PC). We further provide methods for extracting and quantifying phospholipids in mammalian systems. For complete details on the use and execution of this protocol, please refer to Fang et al. (2022).

A review of non-point source water pollution modeling for the urban-rural transitional areas of China: Research status and prospect.

China has experienced a rapid period of urbanization since the 1980s. Many traditional agricultural areas were transformed into the urban-rural transitional areas, in which both urban and rural characteristics exist. Non-point source pollution (NPSP) has become a major side effect of urbanization and agricultural production which caused wide public concerns. It is crucial to carry out research on identifying the spatiotemporal variation in NPSP in the urban-rural transitional area (especially in developing countries, e.g., in China), which is a prerequisite for improving water quality and guiding NPSP control efforts. Modeling approaches are great tools to provide quantitative information on NPSP and optimize the best management practices for NPSP control. We reviewed over twenty years of publications on NPSP modeling and applications in urban, rural and its transitional areas. The strengths and limitations of 20 commonly used NPSP models in China were concluded based on a brief introduction and the evolution history. Reporting the strengths and weaknesses of each NPSP model could enhance its utility in practice. In terms of the unique characteristics of urban-rural transitional areas, which are neither strictly urban nor rural, non-point source pollutants are often distinctly different between traditional pollutants from urban and agricultural areas since the great differences in the hydrological processes, and none of existing NPSP models are fully applicable to urban-rural transitional areas. Based on limited NPSP modeling studies in urban-rural transitional areas, the existing research insufficiency were technical and mechanism limitations of the model despite of numerous improvements in the past, concerns about simulation accuracy, limited investigations on new pollutants, and lack of monitoring data. Future development trend and concerns of NPSP models for urban-rural transitional areas were discussed, which could be of great help to the development of NPSP models and their applications in water quality management in the rapid urbanized China.

Biosorption and biomineralization of U(VI) by Kocuria rosea: Involvement of phosphorus and formation of U-P minerals.

The biosorption and biomineralization behavior of U(VI) by Kocuria rosea with uranium resistance higher than other general microorganisms was investigated in this study. The results showed the obvious effects of initial U(VI) concentration, biomass, time, and especially pH, and presented that U(VI) was immobilized to K. rosea by physical and chemical action. The characterization results for the precipitation proved that U-P minerals with U(VI) (H3OUO2PO4·3H2O, H2(UO2)2(PO4)2·8H2O) or U(IV) (CaU(PO4)2) were dominant, and the crystallization level increased with time. In the process, the phosphorous containing groups, amino, hydroxyl and carboxyl groups played important roles in adsorption of U(VI), and the phosphate groups were crucial in immobilization of uranium, showing the importance of groups containing phosphorus in both biosorption and biomineralization processes. Our findings focus on the biosorption and biomineralization mechanism of U(VI) by K. rosea, emphasize the synergy of physical adsorption and chemical immobilization in the process and formation of U(VI)-P and U(IV)-P minerals, and highlight the significance of phosphorus involvement in the reaction.

The role of brain metastases in patients with pulmonary adenocarcinoma: a retrospective study.

BACKGROUND: Brain metastasis is common in patients with lung cancer and has a negative impact on overall survival (OS). The aim of this study was to investigate therapeutic effects and prognosis-related factors for survival of patients with pulmonary adenocarcinoma and brain metastasis. METHODS: This was a retrospective study involving the data of patients with pulmonary adenocarcinoma and brain metastasis who were treated at the Department of Oncology, Tianjin Medical University General Hospital. Participants were divided into 2 groups: the initial-metastasis group (brain metastases at first visit), and the late-metastasis group (developed metastasis during treatment). The quantitative index was described as mean ± standard deviation (SD), independent sample t-test was used to calculate the P value. All data were processed using the software SPSS 20.0 software package. RESULTS: There were a total of 43 patients in the initial-metastasis group and 49 in the late-metastasis group. First-line treatment efficacy (P=0.004), OS (P<0.001), and progression-free survival (PFS) (P=0.006) were higher in the late-metastasis group. Kaplan-Meier curves revealed that the late-metastasis group had longer OS, intracranial progression-free survival (IPFS), and PFS (all P<0.001). Multivariate analysis showed that gender, Karnofsky performance status (KPS), efficacy of first-line treatment, radiotherapy, and brain metastasis at initial visit were significantly related to OS. CONCLUSIONS: Participants with pulmonary adenocarcinoma and brain metastasis at their initial visit had shorter OS and PFS than those who developed brain metastasis later. The efficacy of first-line treatment was related to OS while female gender and high KPS score were protective factors.

Dynamics and Endocytosis of Flot1 in Arabidopsis Require CPI1 Function.

Membrane microdomains are nano-scale domains (10-200 nm) enriched in sterols and sphingolipids. They have many important biological functions, including vesicle transport, endocytosis, and pathogen invasion. A previous study reported that the membrane microdomain-associated protein Flotillin1 (Flot1) was involved in plant development in Arabidopsis thaliana; however, whether sterols affect the plant immunity conveyed by Flot1 is unknown. Here, we showed that the root length in sterol-deficient cyclopropylsterol isomerase 1 (cpi1-1) mutants expressing Flot1 was significantly shorter than in control seedlings. The cotyledon epidermal cells in cpi1-1 mutants expressing Flot1 were smaller than in controls. Moreover, variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) and single-particle tracking (SPT) analysis demonstrated that the long-distance Flot1-GFP movement was decreased significantly in cpi1-1 mutants compared with the control seedlings. Meanwhile, the value of the diffusion coefficient G was dramatically decreased in cpi1-1 mutants after flagelin22 (flg22) treatment compared with the control seedlings, indicating that sterols affect the lateral mobility of Flot1-GFP within the plasma membrane. Importantly, using confocal microscopy, we determined that the endocytosis of Flot1-GFP was decreased in cpi1-1 mutants, which was confirmed by fluorescence cross spectroscopy (FCS) analysis. Hence, these results demonstrate that sterol composition plays a critical role in the plant defense responses of Flot1.

Untangling the effects of shallow groundwater and deficit irrigation on irrigation water productivity in arid region: New conceptual model.

Water scarcity and salt stress are two main limitations for agricultural production. Groundwater evapotranspiration (ETg) with upward salt movement plays an important role in crop water use and water productivity in arid regions, and it can compensate the impact of deficit irrigation on crop production. Thus, comprehensive impacts of shallow groundwater and deficit irrigation on crop water use results in an improvement of irrigation water productivity (IWP). However, it is difficult to quantify the effects of groundwater and deficit irrigation on IWP. In this study, we built an IWP evaluation model coupled with a water and salt balance model and a crop yield estimation model. As a valuable tool of IWP simulation, the calibrated model was used to investigate the coupling response of sunflower IWP to irrigation water depths (IWDs), groundwater table depth (GTDs) and groundwater salinities (GSs). A total of 210 scenarios were run in which five irrigation water depths (IWDs) and seven groundwater table depths (GTDs) and six groundwater salinities (GSs) were used. Results indicate that increasing GS clearly increases the negative effect on a crop's actual evapotranspiration (ETa) as salt accumulation in root zone. When GS is low (0.5-1g/L), increasing GTD produces more positive effect than negative effect. In regard to relatively high GS (2-5g/L), the negative effect of shallow-saline groundwater reaches a maximum at 2m GTD. Additionally, the salt concentration in the root zone maximizes its value at 2.0m GTD. In most cases, increasing GTD and GS reduces the benefits of irrigation water and IWP. The IWP increases with decreasing irrigation water. Overall, in arid regions, capillary rise of shallow groundwater can compensate for the lack of irrigation water and improve IWP. By improving irrigation schedules and taking advantages of shallow saline groundwater, we can obtain higher IWP.

[Localization of epileptic foci in frontal lobe epilepsy and its surgical therapy].

OBJECTIVE: To analyze the clinical data and pre-operative examination results of frontal lobe epilepsy and combine with intra-operative intracranial electrical record in order to localize epileptic foci and to direct surgical therapy. METHODS: Preoperative EEG record and MRI scan were performed in 23 patients with refractory frontal lobe epilepsy. Among them, 17 patients received interictal 18F-FDG-PET-CT, 11 received MEG examination, 2 received functional MRI, 10 received surgical operation and intra-operative intracranial electrical record. RESULTS: The positive predictive value of clinical features of epileptic seizure, interictal EEG, ictal EEG, MRI and PET-CT were 56.52%, 56.52%, 60.87%, 54.55% and 94.12% respectively. Their consistent results helped to confirm the epileptogenic zone. MEG was more accurate than EEG. ECoE and VEEG monitoring was significant for operative guidance. CONCLUSION: A series of examinations is necessary for the diagnosis of epileptogenic zone of frontal lobe epilepsy. The surgical outcome is related to the accuracy of epileptic foci localization and the removal of epileptogenic zone.